Opening of the Neuro Meetups Bern 2019: Multiple Sclerosis
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About The Event
Ultrastructural 3D analysis of T-cell diapedesis across the Blood-Brain Barrier
Mariana Dias, PhD student at Engelhardt lab, TKI, University of Bern
In multiple sclerosis, autoaggressive T cells cross the blood-brain barrier (BBB) and enter the CNS parenchyma promoting inflammation, demyelination and eventually neurodegeneration. T-cell diapedesis across the BBB can occur via a paracellular pathway across the endothelial junctions or via a transcellular pathway, through a pore across the endothelial cell body. However, the precise mechanisms underlying paracellular versus transcellular T-cell diapedesis across the BBB remain to be explored. Thus, the aim of the present study is to determine the subcellular structures formed during T-cell interaction with the BBB. To do so, we combined in vitro live cell imaging and serial block face scanning electron microscopy (SBF-SEM), to perform the analysis in a 3D ultrastructural level.
Nano-scale microfluidics to study 3D chemotaxis at the single cell level
Corina Frick, PhD student at Mehling lab, Department of Biomedicine, University of Basel
Chemotaxis of immune cells plays an important role in immune surveillance and inflammation. The mode of migration depends highly on microenvironmental factors such as exposure to 2D surfaces or 3D matrices. We have developed a microfluidic migration device which allows to study immune cell migration in a 3D collagen environment in highly controlled diffusion-based chemokine gradients.